Table 1

Bacterial strains used in this studya

Strain or mutationGenotypeReference or source
Strains
    PAO1Wild-type P. aeruginosaR. E. Hancock
    PA4773PA4773 transposon mutant, nontranscriptional fusion, 98_E137
    PA4773-4::luxIntergenic PA4773-PA4774 transposon mutant, and transcriptional fusion, 11_A943
    PA4774::luxPA4774::lux transposon mutant and transcriptional fusion, 38_F937
    PA4775::luxPA4775::lux transposon mutant and transcriptional fusion, 50_F537
    PA4774::luxCCPA4774::lux chromosomally complemented with PA4773-PA4775 integrated into attTn7 siteThis study
    PA4773-4::lux phoQ::xylEDouble mutant; transcriptional fusion PA4773-4::lux in a phoQ::xylE mutant background43
    PA4773-4::lux pmrB::xylEDouble mutant; transcriptional fusion PA4773-4::lux in a pmrB::xylE mutant background43
    PA3553::luxPA3553::lux transposon mutant and transcriptional fusion, 53_D1037
Plasmids
    pUCP22Ampr, multicopy broad-host-range expression vector64a
    pCR2.1-TOPOPCR cloning vector, AmprInvitrogen
    pUC18T-mini-Tn7T-GmGmr on mini-Tn7T; mobilizable; for gene insertion in Gms bacteria10
    pTNS2Helper plasmid for integration into the att site10
    pUC18T-mini-Tn7T-PA4773-PA4775pUC18T-mini-Tn7 containing PA4773-PA4775 genes, including native upstream promoterThis study
  • a Abbreviations: Ampr, ampicillin resistance; Gmr, gentamicin resistance; Gms, gentamicin sensitive.