TABLE 2

Functional roles of genes involved in hexuronate/aldarate utilization in proteobacteria

ProteinaRole
Regulators
    GguR*Predicted transcriptional regulator for hexuronate/aldarate utilization,
GntR family
    GudR*Predicted transcriptional regulator for glucarate utilization, LacI family
    GulR*Predicted transcriptional regulator for hexuronate/aldarate utilization,
LysR family
    UdhR*Predicted transcriptional regulator for hexuronate utilization, LacI family
Enzymes
    UdhUronate dehydrogenase (EC 1.1.1.203)
    UxuL*Predicted uronate lactonase, PF08450 family
    UxuF*Predicted uronate lactonase, PF10282 family
    GciGalactarolactone cycloisomerase (EC 5.5.1.27)
    GliGalactarolactone isomerase (EC 5.4.1.4)
    GarDmeso-Galactarate dehydratase (EC 4.2.1.42)
    GudDd-Glucarate dehydratase (EC 4.2.1.40)
    KdgD5-Dehydro-4-deoxyglucarate dehydratase (EC 4.2.1.41)
    KgsDAlpha-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26)
    KgsD2*Alpha-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26), alternative
    GarL2-Dehydro-3-deoxyglucarate aldolase (EC 4.1.2.20)
    GarR2-Hydroxy-3-oxopropionate reductase (EC 1.1.1.60)
Transporters
    UxuPQMHexuronate transporter, TRAP family
    TctC*Substrate binding protein of predicted aldarate transporter, TTT family
    GarP (GudP)d-Glucarate/meso-galactarate (aldarate) permease, MFS superfamily
    GguT*Predicted aldarate transporter, MFS superfamily
    ExuTHexuronate transporter, MFS superfamily
    ExuT-II*Predicted hexuronate transporter, MFS superfamily
Additional
    PglPolygalacturonase (EC 3.2.1.15), GH28 family
    AglAlpha-glucosidase (EC 3.2.1.20), GH31 family
    Omp*Outer membrane porin precursor, possibly involved in polygalacturonate
uptake
    AldE*d-Glucuronate/d-galacturonate mutarotase
  • a Proteins with novel functional roles predicted in this work are marked with an asterisk; proteins whose functions were experimentally validated in this study are underlined.