TABLE 1

Kinetic parameters of the hexuronic acid catabolic lactonases UxuF and UxuL

Gene locus tagOrganismd-Galactaro-1,5-lactoned-Glucaro-1,5-lactone
kcat (s−1)Km (mM)kcat/Km (M−1 s−1)kcat (s−1)Km (mM)kcat/Km (M−1 s−1)
UxuL (PF08450)
    Rpic_4446Ralstonia pickettii 12J720 ± 601.1 ± 0.36.5 × 105310 ± 300.88 ± 0.33.5 × 105
    PSPTO_1052Pseudomonas syringae pv.
tomato DC3000
7.0 × 1052.2 × 106
UxuF (PF10282)
    Bcep1808_2255Burkholderia vietnamiensis G43.7 × 1064.1 × 105
    BMULJ_02167Burkholderia multivorans
ATCC 17616
4.5 × 1064.2 × 105
    Bcep18194_A5499Burkholderia lata ATCC 177605.9 × 1053.4 × 105
    PSPTO_2765Pseudomonas syringae pv.
tomato DC3000
3.2 × 1067.0 × 104