TABLE 1

Photoheterotrophic growth of Rhodobacter sphaeroides with 3-hydroxypropionate or succinate as the carbon source

StrainaDoubling time (h) withb:
3-HydroxypropionateSuccinate
Rhodobacter sphaeroides 2.4.15.5 ± 0.6 (1.6)3.3 ± 0.4
WT (pBBR)6.6 ± 0.43.5 ± 0.1
WT (ccr)6.2 ± 0.53.8 ± 0.3
WT (ecm)6.3 ± 0.34.0 ± 0.8
WT (mcd)6.7 ± 0.73.6 ± 0.5
WT (mch)6.7 ± 0.93.7 ± 0.4
WT (mcl1)6.3 ± 0.33.6 ± 0.4
WT (ccr mch)6.6 ± 0.63.8 ± 0.1
WT (yciA)9.5 ± 0.4 (1.4)4.5 ± 0.3
RsΔccr23KB6.1 ± 0.43.1 ± 0.3
RsΔccr23KB (pBBR)8.1 ±1.23.4 ± 0.3
RsΔccr23KB (ccr)6.2 ± 1.13.2 ± 0.4
RsΔccr23KB (ccr mch)7.2 ± 0.63.6 ± 0
RsΔecm47KBNE (0.71)3.8 ± 0.3
RsΔecm47KB (pBBR)NE (0.41)3.7 ± 0.6
RsΔecm47KB (ecm)6.4 ± 0.43.9 ± 0.8
RsΔmcd11KBNE (0.18)3.7 ± 0.1
RsΔmcd11KB (pBBR)NE (0.13)3.5 ± 0.2
RsΔmcd11KB (mcd)6.2 ± 0.73.9 ± 0.8
RsΔmch49KBNG3.1 ± 0.2
RsΔmch49KB (pBBR)NG3.5 ± 0.2
RsΔmch49KB (mch)6.7 ± 0.93.6 ± 0.5
RsΔmch49KB (ccr mch)7.4 ± 0.93.8 ± 0.1
RsΔmch49KB (yciA)31 ± 3 (0.29)4.7 ± 0.8
RsΔmcl1_4KBNG3.7 ± 0.1
RsΔmcl1_4KB (pBBR)NG3.8 ± 0.4
RsΔmcl1_4KB (mcl1)6.3 ± 0.63.9 ± 0.6
RsΔccrΔmch24AF7.5 ± 0.63.3 ± 0.5
RsΔccrΔmch24AF (pBBR)8.2 ± 0.33.4 ± 0.4
RsΔccrΔmch24AF (ccr)NG4.1 ± 0.4
RsΔccrΔmch24AF (mch)7.8 ± 0.93.8 ± 0.2
RsΔccrΔmch24AF (ccr mch)6.8 ± 0.33.7 ± 0.4
  • a WT, wild type.

  • b Standard deviation was calculated using three or more biological replicates. The average OD578 after 100 h of growth is given in parentheses. NE, nonexponential growth; NG, no growth.