TABLE 1

Genes highlighted by TraDIS as providing a fitness benefit to UPEC ST131 in the presence of colicinsa

Gene nameFunction of productlogFCbq valueAccession no.
0061LPS core heptosyltransferase−12.1491.06E−235WP_001236430.1
rfaG_1LPS core glycosyltransferase−2.0884.55E−029WP_000634259.1
rfaHTranscription factor involved in LPS biosynthesis−4.8421.10E−056WP_001192400.1
rfaJ_2LPS core glycosyltransferase−8.3540WP_000376840.1
rfaJ_1LPS core 3-alphagalactosyltransferase−7.0050WP_001188027.1
rfaLO-antigen ligase−5.2970WP_000958045.1
rfbCRhamnose-specific dTDP-4-dehydrorhamnose-3,5-epimerase−11.9672.07E−158WP_001100793.1
lacA_1WbbJ; catalyzes the transfer of the O-acetyl moiety to the O-antigen−10.9531.24E−105WP_001296213.1
rfbDdTDP-glucose-4,6-dehydratase−10.9190WP_000699407.1
wbbLGlycosyltransferase−10.6519.64E−080WP_000262536.1
rfaG_2LPS core glycosyltransferase−9.9779.71E−214WP_001296214.1
01679Hypothetical protein−9.1901.26E−153WP_000027654.1
01681Glycosyltransferase−6.9852.70E−036WP_000517629.1
rfbBRhamnose-specific dTDP-glucose-4,6-dehydratase−3.5450WP_000699407.1
rmlA1Rhamnose-specific glucose-1-phosphate thymidylyltransferase−2.9800WP_000857525.1
wecAECA transferase−7.9150WP_001050960.1
04729Putative common enterobacterial antigen polymerase−7.3605.76E−048WP_000055112.1
wzxEECA flippase−4.5471.14E−064WP_000050265.1
arnELipid A modification (CAMP resistance) flippase subunit−2.4131.86E−021WP_000638016.1
arnDLipid A modification (CAMP resistance) deformylase−2.2711.82E−080WP_000169713.1
02987Hypothetical protein−1.9609.74E−009WP_001295929.1
  • a Results are in the presence of EDTA.

  • b Differences in insertions between the control and colicin-treated sample are measured as a log fold change (logFC).