TABLE 1

HPLC and GC-MS analyses of culture supernatants from cells grown by anaerobic respiration with DMSO in the darka

Strain1,2-PD added (mM)1,2-PD consumed (mM)bProduct formed (mM)cCarbon recovered in excreted products (%)d
PropionaldehydePropionatePropanol
SB10030NDNDNDNDND
SB10032013.9 ± 0.49.0 ± 0.91.5 ± 0.02.0 ± 0.290 ± 7
Δ2211200.2 ± 0.2eNDeNDfNDfND
Δ22142014.0 ± 0.913.3 ± 1.70.2 ± 0.0f0.6 ± 0.0101 ± 14
Δ2198201.0 ± 1.3eNMNMNMNM
  • a Cells were grown for 48 h in minimal medium containing pyruvate and glucose as carbon sources, with or without 1,2-PD. Values represent the average of at least 3 replicates, and errors represent standard deviations. The t tests were conducted on samples and compared to SB1003 cells grown in the presence of 1,2-PD. ND, not detected; NM, not measured.

  • b Measured with HPLC.

  • c Measured with GC-MS.

  • d Sum of carbon in excreted products divided by 1,2-PD carbon consumed; this assumes that all carbon in excreted products is derived from 1,2-PD.

  • e P < 0.001.

  • f P < 0.01.