Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionaReference or source
C. rodentium strains
    DBS100Wild type46
    ΔpmrABDBS100 ΔpmrAB31
    Δpmrc ΔcptADBS100 ΔpmrC ΔcptA31
    ΔpmrCDBS100 ΔpmrC31
    ΔcptADBS100 ΔcptA31
    ΔcroPDBS100 ΔcroP47
    pWSK129Low-copy-number cloning vector, Kanr48
    pACYC184Low-copy-number cloning vector, CmrNEB
    pWSKpmrCpmrC cloned into pWSK129 (native promoter)31

cptA cloned into pWSK129 (native promoter)31
    pACYCcptAcptA cloned into pACYC184 (native promoter)This study
    pWSKpmrABpmrAB cloned into pWSK129 (under control of constitutive promoter at the 3′ end of the pmrC coding region)31
  • a Kanr, kanamycin resistance; Cmr, chloramphenicol resistance; NEB, New England BioLabs.