TABLE 2

Oligonucleotides used in this study

Primer nameaSequence (5′ to 3′)bPurpose
acs1P1GTTACCGACT CGCATCGGGC AATTGTGGGT TACGATGGCA TCGCGATAGC ATTCCGGGGA TCCGTCGACCConstruction
acs2P2AACGCTTATG CCACATATTA TTAACATCCT ACAAGGAGAA CAAAAGCATG TGTAGGCTGG AGCTGCTTCGConstruction
acs-Right-PCR-verifAAAACTGCCA ATACCCCTVerification
acs-Left-PCR-verifTTTTAATTCC CGCTCCCTVerification
ackA-pta-Left-PrimerTGGCTCCCTG ACGTTTTTTT AGCCACGTAT CAATTATAGG TACTTCCATG ATTCCGGGGA TCCGTCGACCConstruction
ackA-pta-Right-PrimerGCAGCGCAAA GCTGCGGATG ATGACGAGAT TACTGCTGCT GTGCAGACTG TGTAGGCTGG AGCTGCTTCGConstruction
ackA-pta-Left-PCR-verifCCCTGACGTT TTTTTAGCCVerification
ackA-pta-Right-PCR-verifCAGCGCAGTT AAGCAAGAVerification
ackA-Right-PCR-verifTATCCTCTTT CGTTACCGCCVerification
pta-Left-PCR-verifGGCGGTAACG AAAGAGGAVerification
poxB-seq-RevCTCCTTTCTC TCCCATCCCVerification
poxB-seq-FwdTAAACGTCGT CCCCAACCVerification
pta-CCDB1CTTTCTAGAG AATAGGAACT TCGAACTGCA GGTCGACGGA TCCCCGGAAT TTATATTCCC CAGAACATCA GGConstruction
ackA-KN1TGGCTCCCTG ACGTTTTTTT AGCCACGTAT CAATTATAGG TACTTCCatg ATAGGAACTT CAAGATCCConstruction
Y2-ACKACTTTCTAGAG AATAGGAACTT CGAACTGCAG GTCGACGGAT CCCCGGAATc acGGTTTATC CTCTTTCGTConstruction
ackA compl verifCGCAAAATGG CATAGACTCA AVerification
  • a Note that ackA-Right-PCR-verif and ackA-pta-Left-PCR-verif were used to verify the ackA deletion, pta-Left-PCR-verif and ackA-pta-Right-PCR-verif were used to verify the pta deletion, and ackA-pta-Right-PCR-verif and ackA-compl-verif were used for verifying the ackA complementation.

  • b The lowercase sequences in ackA-KN1 and Y2-ACKA represent the start codons of the genes ackA and pta, respectively.