Table 1

Bacterial strains and plasmids used in this study

Bacterial strain or plasmidRelevant genotype or phenotype or descriptionaSource or reference
E. coli DH5αfhuA2 Δ(argF-lacZ)U169 phoA glnV44 ϕ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17Stratagene
M. smegmatis
    mc2155Electrocompetent wild-type strain of M. smegmatis56
    JR121mc2155 ΔvapBC::aphA-3; Kmr51
Plasmids
    pDEST17Gateway expression vector for M. smegmatis; HygrLab stock
    pcDNA3Mammalian expression vector; AprInvitrogen
    pGEM-T EasyCloning vector; AprPromega
    pJR29pMind harboring vapC with synthetic RBS from kanamycin marker; Kmr Hygr51
    pJR230pMind harboring vapBC with synthetic RBS from kanamycin marker; Kmr Hygr51
  • a RBS, ribosome binding site.