Table 1

Anabaena strains

Strain/genotypeaDerivation/relevant characteristicsbReference or source
Anabaena sp. PCC 7120Wild typeFACHBc
DRHB213Nmr, patA::C.K2d with neomycin resistance cassette C.K2d inserted into the EcoRV site of patA, downstream of which is a gene in the opposite direction; C.K2d is essentially the same as C.K2 (10) except for a 244-bp deletion downstream of the neomycin resistance gene39
DRHB213 (pHB1123)Nmr Spr, pHB1123 carrying PhetR-gfp in pHB912 (31) introduced into the patA::C.K2d mutantThis study
DRHB213 (pHB1465)Spr Nmr, pHB1465 carrying PhetZ-gfp in the pDU1-based high-copy-no. plasmid pHB912, introduced into the patA::C.K2d mutant39
DRHB213 (pHB3103)Nmr Spr, pHB3103 carrying Pall0097 (chromosomal DNA bp 103174-103769) upstream of gfp in pHB912 introduced into the patA::C.K2d mutantThis study
DRHB213 (pHB3706)Nmr Spr, pHB3706 carrying PhetR-hetR-gfp encoding HetR-GFP in pRL25C introduced into the patA::C.K2d mutantThis study
DRHB214Nmr, hetZ::C.K2 with neomycin resistance cassette C.K2 inserted into the EcoRV site of hetZ, downstream of which is patU3, which can be independently transcribed39
DRHB214 (pHB1465)Spr Nmr, pHB1465 carrying PhetZ-gfp in pHB912 introduced into the hetZ::C.K2 mutant39
DRHB2458Emr, hetR::C.CE2 with chloramphenicol/erythromycin resistance cassette C.CE2 (2) inserted into the ClaI site of hetR, downstream of which is a gene in the opposite directionThis study
DRHB2458 (pHB4024)Emr Spr, pHB4024 carrying PhetR-tsf-hetR in pRL25C introduced into the hetR::C.CE2 mutantThis study
hetF::Tn5-1087bEmr, Tn5-1087b inserted within hetF 310 bp away from its 3′ terminus22
hetF::Tn5-1087b (pHB1123)Emr Spr, pHB1123 carrying PhetR-gfp in pHB912 introduced into the hetF mutantThis study
hetF::Tn5-1087b (pHB1127)Emr Spr, pHB1127 carrying PpatA-gfp in pHB912 introduced into the hetF mutantThis study
hetF::Tn5-1087b (pHB1465)Emr Spr, pHB1465 carrying PhetZ-gfp in pHB912 introduced into the hetF mutantThis study
hetF::Tn5-1087b (pHB3271, pHB1123)Emr Spr, pHB1123 carrying PhetR-gfp in pHB912 introduced into hetF::Tn5-1087b (pHB3271)dThis study
hetF::Tn5-1087b (pHB3271, pHB1127)Emr Spr, pHB1127 carrying PpatA-gfp in pHB912 introduced into hetF::Tn5-1087b (pHB3271)This study
hetF::Tn5-1087b (pHB3271, pHB1465)Emr Nmr Spr, pHB1465 carrying PhetZ-gfp in pHB912 introduced into hetF::Tn5-1087b (pHB3271)This study
WT (pHB1465)Spr, pHB1465 carrying PhetZ-gfp in pHB912 introduced into Anabaena 712039
WT (pHB3103)Spr, pHB3103 carrying Pall0097 (chromosomal bp 103769-103174) upstream of gfp in pHB912 introduced into Anabaena 7120This study
WT (pHB3106)Spr, pHB3106 carrying PhetZ-1 (chromosomal bp 102472-103037) upstream of gfp in pHB912 introduced into Anabaena 7120This study
WT (pHB3107)Spr, pHB3107 carrying PhetZ-2 (chromosomal bp 102567-103037) upstream of gfp in pHB912 introduced into Anabaena 7120This study
WT (pHB3108)Spr, pHB3108 carrying PhetZ-3 (chromosomal bp 102668-103037) upstream of gfp in pHB912 introduced into Anabaena 7120This study
WT (pHB3109)Spr, pHB3109 carrying PhetZ-4 (chromosomal bp 102825-103037) upstream of gfp in pHB912 introduced into Anabaena 7120This study
WT (pHB3110)Spr, pHB3110 carrying PhetZ-5 (chromosomal bp 102914-103037) upstream of gfp in pHB912 introduced into Anabaena 7120This study
WT (pHB3224)Spr, pHB3224 carrying PhetZ-6 (chromosomal bp 102668-102934) upstream of gfp in pHB912 introduced into Anabaena 7120This study
WT (pHB4067)Spr, pHB4067 carrying PhetZ-5-1 (chromosomal bp102914-103038, with bp 102966-103016 deleted) upstream of gfp in pHB912 introduced into Anabaena 7120This study
WT (pHB4114)Spr, pHB4114 carrying PhetZ-8 (chromosomal bp 102668-103720) upstream of gfp in pHB912 introduced into Anabaena 7120This study
WT (pHB4115)Spr, pHB4115 carrying PhetZ-7 (chromosomal bp 102668-103395) upstream of gfp in pHB912 introduced into Anabaena 7120This study
  • a DRHB (number) refers to a product of double homologous recombination between plasmid pHB (number) and the Anabaena 7120 genome; the plasmids are described in detail in Table S1 in the supplemental material. WT, wild type.

  • b Ap, ampicillin; Em, erythromycin; Km, kanamycin; Sm, streptomycin; Sp, spectinomycin. Unless stated otherwise, the template for PCRs was Anabaena 7120 genomic DNA.

  • c FACHB, Freshwater Algal Culture Collection of the Institute of Hydrobiology, Chinese Academy of Sciences.

  • d The plasmid pHB3271 carries PpsbA-hetR.