Table 2

Suppressors of eutD

GenotypeDNA sequence changeEffect on proteinDoubling time on ethanolamine (h/generation)Relative fitnessa
Wild type (TR10000)No change (eut+)3.91.00
eutD372In-frame deletion of eut-coding sequenceNo EutD protein>16<0.24
Extended deletions that restore growth on ethanolamine to eutD deletion mutant
    eutQTDMN387Deletes eutQ bp 471 to 8 bp before eutE; removes eutTDMNTruncated EutQ, no EutTDMN; wild-type EutE3.41.14
    eutQTDMNE388Deletes eutQ bp 343 to eutE bp 51; removes eutTDMNFuses EutQ to EutE (−17AA); EutE+3.90.99
Single gene mutations that restore growth on ethanolamine to a eutD deletion mutant
    eutQ386C94AP32T substitution4.60.84
    eutM389G13AG5R substitution3.71.05
    eutM390A25CT9P substitution4.00.97
    eutM391C26AT9N substitution4.00.97
    eutM392Deletion of bp 80–94 (15 bp), 9-bp join pointInternal deletion of 5 amino acids3.71.05
    eutM393Deletion of bp 81–92 (12 bp), 2-bp join pointInternal deletion of 4 amino acids3.51.09
    eutM394Duplication of bp 117–121, 5-bp insertionFrameshift, premature termination3.81.00
    eutM395Deletion of bp 137–144 (8 bp), 0-bp join pointDeletion, frameshift, premature termination3.61.06
    eutM396G142TD48Y substitution3.61.06
    eutM397C152TA51V substitution4.30.90
    eutM398Deletion of bp 176–183 (8 bp), 3-bp join pointDeletion, frameshift, premature termination3.61.06
    eutM399Deletion of bp 191; G192C, C193A, T198CFrameshift, premature termination3.61.07
    eutM400G250TNonsense mutation. premature termination3.51.02
    eutE401G19AE7K substitution in signal sequence3.61.07
    eutE402T38AL13Q substitution in signal sequence3.31.17
  • a Fitness expressed as the ratio of the wild-type doubling time to that of the mutants.