Table 5

Effect of eut deletion mutations on microcompartment integrity as judged by acetate growth

GenotypeaStrainDoubling time (h/generation)Relative fitnessbMicrocompartment disruption
Wild typeTT26709>16<0.23
eutSΔ eutMNΔ eutLKΔTT267103.691.00+
eutMNΔ eutLKΔTT267113.970.93+
eutMΔTT267133.631.02+
eutNΔTT267144.600.80+
eutKΔTT267164.720.78+
eutLΔTT267155.470.67±
eutBΔTT267186.070.61±
eutCΔTT267195.700.65±
eutEΔTT267215.510.67±
eutJΔTT267265.780.64±
eutSΔTT267129.000.41
eutQΔTT267247.950.46
eutPΔTT2672311.520.32
eutTΔTT2672511.500.32
eutAΔTT26717>16<0.23
eutGΔTT26722>16<0.23
eutDΔTT26720>16<0.23
eutDMΔTT26727>16<0.23+
  • a All strains were pta acs mutants.

  • b Relative fitness was scaled to the doubling time of the eutSΔ eutMNΔ eutLKΔ mutant grown with acetate and ethylenediamine, whose fitness was defined to be equal to 1.