Table 6

Effect of eut mutations on ability to grow on ethanol

Line no.GenotypeStrainDoubling time (h/generation)Relative fitnessaMicrocompartment disruption
1Wild typeTR10000>16<0.23
2eutSΔ eutMNΔ eutLKΔTT266803.61.00+
3eutMNΔ eutLKΔTT266793.90.94+
4eutMNEΔTT26730>16<0.23No EutE activity
5eutEΔTT22815>16<0.23No EutE activity
6eutGΔTT23036>16<0.23
7eutMΔ eutGΔTT267294.60.80+
8eutMΔ eutGΔ adhPTT26737>16<0.23No EutG or AdhP dehydrogenase
9eutSΔ eutMNΔ eutLKΔ adhPTT26738>16<0.23No AdhP dehydrogenase
10eutDΔTT24804>16<0.23
11eutDΔ eutMΔTT266943.51.03+
12eutDΔ eutE (signal sequence)TT267003.61.00+
13eutQΔTT248025.10.72+
14eutMΔTT225234.30.85+
15eutLΔTT225704.10.88+
16eutSΔTT225229.60.38
17eutKΔTT22571>16<0.23
18eutNΔTT22569>16<0.23
19eutAΔTT2480514.70.25
20eutBΔTT24806>16<0.23
21eutCΔTT24807>16<0.23
22eutTΔTT24803>16<0.23
23eutPΔTT24801>6<0.23
24eutJΔTT26728>16<0.23
  • a Fitness is the ratio of the doubling time for the eutSΔ eutMNΔ eutLKΔ mutant to that of the other strains.