Table 7

Role of various proteins on compartment integrity as judged by three assays

Mutation addedGrowth ofa:
eutD mutant on ethanolamine with release of acetyl-CoA or EutEpta acs mutant on acetate with release of acetyl-CoA or EutDWild type (adhP+) on ethanol with entry of acetaldehyde or release of EutE
None (wild type)
eutM+++
eutL+±+
eutK++
eutNND+
eutQ++
eutS±
eutE*±*
eutE (signal)b+ND+
eutB*±
eutC*±
eutD*
eutG
eutJ±
eutP
eutTND
eutA*
  • a +, the added mutation disrupts the compartment as judged by this assay; ±, the added mutation leaves compartment intact as judged by this assay; −, the added mutation partially disrupts the compartment; ND, the added mutation was not tested in this assay; *, this protein is needed for growth in this assay and could not be tested for its contribution to the compartment.

  • b Signal sequence mutation.