Table 1

List of bacterial strains used in this study

StrainDescriptionSource or reference
Deinococcus radiodurans R1Wild type (ATCC 13939)20
D. radiodurans FrnEATCC 13939 DR_0659::nptII (frnE::nptII)This study
Escherichia coli DH5αλ φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK mK) supE44 thi-1 gyrA relA1Lab collection
E. coli BL21(DE3) pLysSF ompT hsdSB(rB mB) gal dcm (DE3), a λ prophage carrying the T7 RNA polymerase gene, pLysSLab collection
E. coli DHB4araD139 (ara-leu)7697 (codB-lac)X74, galE15 galK16 rpsL150 relA1 thi phoA (PvuII) phoR malF3 Flac(lacI) pro Strr29
E. coli MB68DHB4 ΔdsbA strain29
E. coli MB69DHB4 ΔdsbC strain29
E. coli MB834DHB4 ΔdsbCD strain29
E. coli NK1E. coli MB68 harboring pMER78This study
E. coli NK2E. coli MB68, ΔdsbA mutant harboring pMERfrnEThis study
E. coli NK3E. coli MB69 harboring pMER78This study
E. coli NK4E. coli MB69 harboring pMERfrnEThis study
E. coli NK5E. coli MB834 harboring pMER78This study
E. coli NK6E. coli MB834 harboring pMERfrnEThis study