Table 1

Strains and plasmids used in this study

Strain or plasmidDescriptionSource or reference
E. coli strains
    DH5αCloning strainGibco
    DH10βCloning strainGibco
    Ec233E. coli/pMT616, Cmr61
    Ec822E. coli/pLW112, Ampr23
    Ec869E. coli/pLW127, Ampr24
    Ec870E. coli/pMF54, Ampr62
    Ec887E. coli/pIM001, AmprThis work
P. aeruginosa strains
    Pa725Strain PAO124
    Pa866Strain PA1441
    Pa867PA14 ΔalgU, Gmr41
    Pa872PA14/pLW127, Cbr AmprThis work
    Pa874PA14/pMF54 Gmr Cbr AmprThis work
    Pa876PA14 ΔalgU/pMF54, Gmr Cbr AmprThis work
    Pa877PA14 ΔalgU/pIM001, Gmr Cbr AmprThis work
    Pa879PA14 ΔpqsA, Gmr41
    Pa891PA14/pLW112, Gmr Cbr AmprThis work
    Pa892PA14/pIM001, Gmr Cbr AmprThis work
    Pa1300PA14 ΔalgD/pMF54, Gmr Cbr AmprThis work
    Pa1301PA14 ΔalgD/pIM001, Gmr Cbr AmprThis work
    Pa1302PA14 ΔwbpM, Gmr41
    Pa1303PA14 ΔwapR, Gmr41
    Pa1304PA14 Δrmd, Gmr41
    Pa1305PA14 ΔalgD, Gmr41
    Pa1312PAO1/pLW112, Gmr Cbr AmprThis work
Plasmids
    pMT616Triparental mating helper plasmid, Cmr61
    pLW127pOsmC::LacZ, AlgU-dependent promoter fused to LacZ, Ampr24
    pMF54pMF54 expression vector, Ampr62
    pLW112pMF54-based MucD expression plasmid, Ampr23
    pIM001pMF54-based expression vector for AlgU, AmprThis work