Table 1

Strains and plasmids

Strain or plasmidRelevant characteristic(s)aReference or source
Plasmids
    pTA131Ampr; pyrE2 under a ferredoxin promoter39
    pTA963Ampr, pyrE2 and hdrB markers, inducible ptna promoter48
    pRE1pTA131 containing chromosomal pilA2 flanking regions 700 bp upstream and 700 bp downstreamThis study
    pRE3pTA963 containing pilA2HisThis study
    pRE8pTA131 containing chromosomal pilA1 flanking regions 700 bp upstream and 700 bp downstreamThis study
    pRE32pTA963 containing pilA3HisThis study
    pRE33pTA963 containing pilA4HisThis study
    pRE34pTA963 containing pilA5HisThis study
    pRE35pTA963 containing pilA6HisThis study
    pRE36pTA963 containing pilA1hybridHisThis study
    pMT1pTA131 containing chromosomal flgA1-flgA2 flanking regions 700 bp upstream and 700 bp downstream37
    pMT24pTA963 containing pilA1HisThis study
Strains
    E. coli
        DH5αF 80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA enda1 hsdR17(rK mK) supE44 thi-1 gyrA relA1Invitrogen
        DL739MC4100 recA dam-13::Tn947
    H. volcanii
        H53ΔpyrE2 Δtrp39
        RE10H53 ΔpilA2This study
        RE12H53 ΔpilA2 ΔpilA3 ΔpilA4 ΔpilA5 ΔpilA6This study
        RE13H53 containing pMT24This study
        RE17MT4 containing MT24This study
        RE19H53 containing pRE3This study
        RE23MT4 containing pRE3This study
        RE27H53 ΔpilA1This study
        RE29H53 ΔpilA1 ΔpilA3 ΔpilA4 ΔpilA5 ΔpilA6This study
        RE30RE43 containing pTA963This study
        RE37H53 containing pRE34This study
        RE38MT4 containing pRE34This study
        RE39MT4 containing pRE35This study
        RE40H53 containing pRE32This study
        RE41H53 containing pRE33This study
        RE42MT4 containing pRE33This study
        RE43H53 ΔpilA1 ΔpilA2 ΔpilA3 ΔpilA4 ΔpilA5 ΔpilA6This study
        RE44RE43 containing pRE34This study
        RE45RE43 containing pMT24This study
        RE46H53 ΔpilA1 ΔpilA2 ΔpilA3 ΔpilA4 ΔpilA5 ΔpilA6 ΔflgA1 ΔflgA2This study
        RE47H53 ΔpilA1 ΔpilA2This study
        RE48H53 containing pRE35This study
        RE49MT4 containing pRE32This study
        RE50RE43 containing pRE35This study
        RE51RE43 containing pRE32This study
        RE52RE43 containing pRE33This study
        RE53RE43 containing pRE3This study
        RE55RE43 containing pRE36This study
        RE58H53 ΔpilA3 ΔpilA4This study
        RE59H53 ΔpilA3 ΔpilA4 ΔpilA5 ΔpilA6This study
        RE61MT4 containing pRE36This study
        RE71RE46 containing pRE36This study
        RE72RE46 containing pMT24This study
        RE73RE46 containing pRE3This study
        RE74RE46 containing pRE32This study
        RE75RE46 containing pRE33This study
        RE76RE46 containing pRE34This study
        RE77RE46 containing pRE35This study
        RE78RE46 containing pTA963This study
        RE79H53 containing pRE36This study
        MT2H53 ΔflgA1 ΔflgA237
        MT4H53 ΔpibD37
        MT13H53 containing pTA96343
        MT44MT2 containing pTA963M. Tripepi, unpublished data
        MT60H53 ΔpilA5 ΔpilA6This study
  • a Ampr, ampicillin resistant.