Table 5

Competition assays to measure the effects of sgrS mutations on growth with αMG in minimal MOPS medium with glycerol

E. coli strain A genotypeE. coli strain B genotypeWithout αMGWith 0.5% αMG
CIaP valuebCIP value
λattB::tetWT0.94 ± 0.04NS1.04 ± 0.06NS
ΔsgrS λattB::tetWT0.98 ± 0.1NS0.38 ± 0.11<0.0001
sgrS1manXYZ λattB::tetWT1.03 ± 0.12NS0.42 ± 0.060.014
sgrS28ptsG λattB::tetWT0.96 ± 0.3NS0.34 ± 0.020.022
sgrS26ptsG,yigL λattB::tetWT0.92 ± 0.07NS0.5 ± 0.030.015
sgrS1manXYZ λattB::tetΔsgrS0.96 ± 0.13NS1.01 ± 0.19NS
sgrS26ptsG,yigL λattB::tetΔsgrS1.1 ± 0.51NS1.86 ± 0.640.023
sgrS28ptsG λattB::tetΔsgrS0.93 ± 0.21NS1.11 ± 0.16NS
sgrS26ptsG,yigL λattB::tetsgrS1manXYZ1.03 ± 0.06NS1.75 ± 0.130.019
sgrS28ptsG λattB::tetsgrS1manXYZ0.91 ± 0.12NS1.02 ± 0.1NS
sgrS26ptsG,yigL λattB::tetsgrS28ptsG1.03 ± 0.11NS2.09 ± 0.250.02
  • a See Materials and Methods for detailed procedures. The competition index (CI) is calculated as follows: (log10 strain A output/log10 strain B output)/(log10 strain A input/log10 strain B input). The data presented are the averages ± standard deviations of three independent experiments.

  • b Student's t test was used to compare the output and inoculum. NS, not significant (P ≥ 0.05).