Table 6

Competition assays to measure the effects of mutations in the three sgrS targets on growth with αMG in minimal MOPS medium with glycerol

E. coli strain A genotypeE. coli strain B genotypeWithout αMGWith 0.5% αMG
CIaP valuebCIP value
ΔptsG λattB::tetWT1.02 ± 0.15NS2.87 ± 0.120.0008
ΔmanXYZ λattB::tetWT0.98 ± 0.11NS1.02 ± 0.008NS
ΔsgrS ΔptsG λattB::tetΔsgrS0.97 ± 0.02NS2.23 ± 0.460.04
ΔsgrS ΔmanXYZ λattB::tetΔsgrS0.97 ± 0.12NS0.97 ± 0.07NS
ΔyigL λattB::tetWT1.01 ± 0.02NS0.26 ± 0.060.031
ΔyigL λattB::tetΔsgrS0.98 ± 0.04NS1.0 ± 0.11NS
  • a See Materials and Methods for detailed procedures. The competition index (CI) is calculated as follows: (log10 strain A output/log10 strain B output)/(log10 strain A input/log10 strain B input). The results presented here are the averages ± standard deviations of three independent experiments.

  • b Student's t test was used to compare the output and inoculum. NS, not significant (P ≥ 0.05).