Table 7

Competition assays to measure the effects of sgrS mutations on growth with 2DG in minimal MOPS medium with fructose

E. coli strain A genotypeE. coli strain B genotypeWithout 2DGWith 0.5% 2DG
CIaP valuebCIP value
λattB::tetWT0.84 ± 0.11NS0.84 ± 0.3NS
ΔsgrS λattB::tetWT1.09 ± 0.14NS0.46 ± 0.10.023
sgrS1manXYZ λattB::tetWT0.97 ± 0.16NS1.03 ± 0.13NS
sgrS28ptsG λattB::tetWT1.00 ± 0.15NS0.68 ± 0.040.01
sgrS1manXYZ λattB::tetΔsgrS0.98 ± 0.22NS2.25 ± 0.590.015
sgrS28ptsG λattB::tetΔsgrS0.96 ± 0.11NS1.12 ± 0.34NS
sgrS28ptsG λattB::tetsgrS1manXYZ1.01 ± 0.06NS0.59 ± 0.080.019
  • a See Materials and Methods for detailed procedures. The competition index (CI) is calculated as follows: (log10 strain A output/log10 strain B output)/(log10 strain A input/log10 strain B input). The results presented here are the averages ± standard deviations of three independent experiments.

  • b Student's t test was used to compare the output and inoculum. NS, not significant (P ≥ 0.05).