Table 1

Bacterial strains and plasmids used in this study

Strain/plasmidGenotype descriptionReference or source
Strains
    86-24Wild-type EHEC (serotype O157:H7)60
    MK3786-24 eutR mutant33
    MK66MK37 with plasmid pMK53This study
    E2348/69Wild-type EPEC (serotype O127:H6)61
    BL-21(DE3)F ompT hsdSB(rB mB) gal dcm (DE3)Invitrogen
Plasmids
    pMAL-c5XCloning/expression vectorNew England Biolabs
    pMK53EHEC 86-24 eutR in pMAL-c5XThis study