Table 3

Hydrolysis of 4-nitrophenyl α-d-mannopyranoside and 4-nitrophenyl β-d-mannopyranoside by P. gingivalis whole-cell suspensions

pHRate of hydrolysis (nmol 4-NP produced/h/OD600 unit of cells)a
α-Mannosidase activityβ-Mannosidase activity
4.5∼0∼0
5.00.02241.519
5.50.1561.959
6.00.2702.171
6.50.2272.154
7.00.1200.681
7.50.0970.167
  • a Rates of hydrolysis were measured in either sodium acetate buffers or Tris-HCl buffers containing 4 mM Ca2+ at 37°C.