Table 4

Hydrolysis of 4-nitrophenyl α-d-mannopyranoside, α-1→2 mannobiose, and α-1→3 mannobiose by cell extracts of P. gingivalis W50 and isogenic mutant strains

P. gingivalis strainRate of hydrolysis of 4-nitrophenyl α-d-mannopyranoside (nmol 4-NP produced/h/ml)a% Activity relative to the parent W50 strainActivity toward α-1→2 mannobiosebActivity toward α-1→3 mannobioseb
W501.564100ActiveActive
ΔPG00321.585102ActiveActive
ΔPG09020.29319ActiveActive
ΔPG09731.628101ActiveActive
ΔPG17111.229101ActiveInactive
ΔPG17121.59676InactiveActive
  • a Rates of hydrolysis were measured at pH 6.0 in 0.1 M sodium acetate buffer containing 4 mM Ca2+ at 37°C.

  • b Hydrolysis of α-1→2 and α-1→3 mannobiose, followed by TLC on Keisel gel plates in n-butanol–ethanol–water (50:50:30, by volume).