Table 2

Bacterial strains used in this study

StrainGenotypeSource or reference
Listeria monocytogenes
    10403S (WT)StrrD. A. Portnoy, lab stock
    ΔmdrM mutantΔmdrM7
    ΔmdrA mutantΔmdrAThis study
    ΔmdrC mutantΔmdrCThis study
    ΔmdrB mutantΔmdrBThis study
    ΔmdrD mutantΔmdrDThis study
    ΔmdrE mutantΔmdrEThis study
    ΔmdrMC mutantΔmdrM ΔmdrCThis study
    ΔmdrMTA mutantΔmdrM ΔmdrT ΔmdrAThis study
    ΔmdrMTAC mutantΔmdrM ΔmdrT ΔmdrA ΔmdrCThis study
    ΔmdrMTAB mutantΔmdrM ΔmdrT ΔmdrA ΔmdrBThis study
    ΔmdrMTAD mutantΔmdrM ΔmdrT ΔmdrA ΔmdrDThis study
    ΔmdrMTAE mutantΔmdrM ΔmdrT ΔmdrA ΔmdrEThis study
    ΔmdrMTABC mutantΔmdrM ΔmdrT ΔmdrA ΔmdrB ΔmdrCThis study
    ΔmdrM mutant (pLIV2-mdrM-6His)ΔmdrM pLIV2 expressing MdrM-6×His7
    ΔmdrM mutant (pLIV2-mdrM-F58V-6His)ΔmdrM pLIV2 expressing MdrM-F58V-6×HisThis study
    ΔmdrMTAC mutant (pLIV2-mdrM-6His)ΔmdrMTAC pLIV2 expressing MdrM-6×HisThis study
    ΔmdrMTAC mutant (pLIV2-mdrT)ΔmdrMTAC pLIV2-mdrTThis study
    ΔmdrMTAC mutant (pLIV2-mdrA)ΔmdrMTAC pLIV2-mdrAThis study
    ΔmdrMTAC mutant (pLIV2-mdrC)ΔmdrMTAC pLIV2-mdrCThis study
    WT (pLIV2-pdeA)pLIV2-pdeAThis study
    ΔmdrMTAC mutant (pLIV2-pdeA)ΔmdrM ΔmdrT ΔmdrA ΔmdrC pLIV2-pdeAThis study
    WT (pLIV2-dacA)pLIV2-dacAThis study
    ΔmdrMTAC mutant (pLIV2-dacA)ΔmdrM ΔmdrT ΔmdrA ΔmdrC pLIV2-dacAThis study
    ΔmarR mutantΔmarR (LMRG_01348.6 [lmo1618])7
    WT (pPL2-PmdrClacZ)10403S harboring integrative plasmid pPL2 containing lacZ gene under mdrC promoterThis study
    ΔmdrMTA mutant (pPL2-PmdrClacZ)10403S ΔmdrMTA harboring integrative plasmid pPL2 containing lacZ gene under mdrC promoterThis study
Escherichia coli
    DH12sϕ80dlacZΔM15 mcrA Δ(mrr-hsdRMS-mcrBC) araD139 Δ(ara leu)7697 Δ(lacX74) galU galK rpsL (Strr) nupG recA1/[F′ proAB+ lacIqZΔM15]
    XL-1brecA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)]Stratagene
    SM-10Conjugation donor; F thi-1 thr-1 leuB6 recA tonA21 lacY1 supE44 (Muc+) λ [RP4-2(Tc::Mu)] Kmr Tra+54