Table 3

Quantification of total and reducing sugars of HMW and LMW EPS-I

EPS-I form (source)aConcn of sugar (μg/ml)Total/reducing sugar ratiob
TotalcReducingd
HMW (exoX strain)4871.4348
LMW (Rm1021)4156.662
  • a Both HMW EPS-I and LMW EPS-I were fractionated by size exclusion chromatography.

  • b High ratios indicate high-molecular-weight EPS-I (longer sugar chains per reducing end). Conversely, low ratios indicate low-molecular-weight EPS-I.

  • c As determined by an anthrone-sulfuric acid assay.

  • d As determined by a neocuproine assay.