Table 1

Strains and plasmids used in this study

Strain or plasmidRelevant characteristicsSource or reference
Salmonella strains
    SJW1103Wild type for motility and chemotaxis33
    SJW2241ΔmotA-motB34
    YSC2123ΔmotA-motB ΔcheY ΔfimA fliC(Δ204-292)This study
    YSC2302ΔmotA-motB ΔfliC::pHluorinThis study
    YVM003gfp-motB18
    YVM012gfp-motB fliC(Δ204-292)This study
    YVM031motA(R90E) gfp-motB18
    YVM032motA(E98K) gfp-motBThis study
    YVM033gfp-motB fliG(D289K)This study
    YVM034gfp-motB fliG(R281V)This study
    YVM035motA(R90E) gfp-motB fliG(D289K)This study
    YVM036motA(E98K) gfp-motB fliG(R281V)This study
    YVM037gfp-motB fliG(R281D)This study
    YVM039motA(E98K) gfp-motB fliG(R281D)This study
    YVM046fliG(R281V)This study
    YVM047motA(E98K)This study
    YVM048motA(E98K) fliG(R281V)This study
Plasmids
    pBAD24Expression vector35
    pYC20pBAD24/MotA+MotB36
    pYC20(R90E)pBAD24/MotA(R90E)+MotB18
    pYC20(E98K)pBAD24/MotA(E98K)+MotB18
    pYC109pBAD24/MotA+MotB(Δ52-71)36
    pYC109(R90E)pBAD24/MotA(R90E)+MotB (Δ52-71)This study
    pYC109(E98K)pBAD24/MotA(E98K)+MotB (Δ52-71)This study