Table 6

agr sequence analysis of hla-negative mutants from 8325, GO, and MMR following 7 days of in vitro passage

Strainagr mutation (gene): mutation typeMutation type aa site: aa(codon)cPredicted result
WT-1agrC(1030): G→T344: Glu(GAA) → stop(TAA)Truncated protein: loss of C-terminal 71 aa of AgrC
WT-2agrA(601): T→C201: Ser(TCG) → Pro(CCG)Ser→Pro at residue 201 of AgrAb
WT-3agrC(1030): G→T344: Glu(GAA) → stop(TAA)Truncated protein: loss of C-terminal 71 aa of AgrC
ΔmutM-1agrC(836): G→T279: Gly(GGC) → Val (GTC)Gly→Val at residue 279 of AgrCb
ΔmutM-2agrC(904): G→T302: Glu(GAA) → stop(TAA)Truncated protein: loss of C-terminal 113 aa of AgrC
ΔmutM-3None detected
ΔmutY-1None detected
ΔmutY-2agrC(692): C→A231: Thr(ACA) → Lys(AAA)Thr→Lys at residue 231 of AgrCb
ΔmutY-3agrC(692): C→A231: Thr(ACA) → Lys(AAA)Thr→Lys at residue 231 of AgrCb
ΔmutS-1agrC(620): A→G207: Tyr(TAT) → Cys(TGT)Tyr→Cys at residue 207 of AgrCb
ΔmutS-2agrC(620): A→G207: Tyr(TAT) → Cys(TGT)Tyr→Cys at residue 207 of AgrCb
ΔmutS-3agrC(793): A→T265: Lys(AAA) → stop(TAA)Truncated protein: loss of C-terminal 150 aa of AgrC
ΔmutL-1agrA(396|397a): T insertionFrameshiftAltered coding sequence occurs within 6T homopolymeric run of agrA; impacts residue 132 within the LytSR DNA binding domain (119–208)
ΔmutL-2agrC(179): G→A179: Trp(TGG) → stop(TAG)Truncated protein: loss of C-terminal 236 aa of AgrC
ΔmutL-3agrC(1189): G→A397: Asp(GAT) → Asn(AAT)Asp→Asn at residue 397 of AgrCb
ΔmutSL-1agrC(252|253a): AT insertionFrameshiftAT Insertion occurs at AT (dinucleotide) triplet repeat (5′-ATATAT) of agrC
ΔmutSL-2agrC(252|253a): AT insertionFrameshiftAT Insertion occurs at AT (dinucleotide) triplet repeat (5′-ATATAT) of agrC
ΔmutSL-3Not determined
  • a Insertion site occurs between the gene nucleotide sites separated by a straight line.

  • b Intolerant amino acid change as determined by the SIFT algorithm (67).

  • c aa, amino acid.