Table 1

Bacterial strains and plasmids

Strain or plasmidDescriptionaReference or source
Strains
    Vibrio alginolyticus
        VIO5VIK4 Rifr Pof+ Laf50
        LPN1VIO5 ΔflhF Rifr Pof+ Laf23
        LPN2VIO5 ΔflhFG Rifr Pof+ Laf23
        LPN1-supMotile revertant of LPN132
        LPN2-supMotile revertant of LPN232
        LPN3VIO5 ΔsflA Rifr Pof+ LafThis study
        LPN4VIO5 ΔflhFG ΔsflA Rifr Pof+ LafThis study
    Escherichia coli DH5αfhuA2 Δ(argF-lacZ)U169 phoA glnV44 ϕ80Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17
Plasmids
    pSU21Cmr41
    pCold IAmprTaKaRa
    pNT30pSU21 with the VEA003729 gene and sflAThis study
    pNT40pSU21 with the VEA003729 gene and sflA-Δ9This study
    pNT31sflA, pNT30 with BamHI and BglII digestThis study
    pNT32VEA003729, pNT30 treated with Eco47IIIThis study
    pNT33sflA, pNT30 treated with NotIThis study
    pNT34VEA003729, pNT30 treated with MluIThis study
    pNT41pSU21, sflAC with a His6 tag at the C terminusThis study
    PNT51pCold I, sflAN with a His6 tag at the N terminusThis study
    pGEM-TAmprPromega
  • a Abbreviations: Rifr, rifampin resistant; Laf, lacking lateral flagella; Pof+, possessing a polar flagellum; Cmr, chloramphenicol resistant; Ampr, ampicillin resistant.