Table 1

Bacterial strains and plasmids used in this study

Strain or plasmidCharacteristicsReference or source
Strains
    E. coli
     HB101F supE44 hsdS20(rB mB) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1B. Dupuy
     DH5αfhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17New England Biolabs, USA
    C. difficile
     JIR8094Ems derivative of strain 63019
     LB-CD4JIR8094 prdB::ermBThis study
     LB-CD12JIR8094 grdA::ermBThis study
     LB-CD8JIR8094 prdR::ermBThis study
     LB-CD13JIR8094 pBL58This study
     LB-CD14LB-CD8 pBL93This study
Plasmids
    pBL33pCE240 targeted to prdBThis study
    pBL64pCR2.1-intron template part AThis study
    pBL65pCR2.1-intron template part BThis study
    pBL67pCE240 targeted to prdRThis study
    pBL68pMC123 without HindIII and BsrGI sitesThis study
    pBL71pMC123 containing groupII intron targeted to prdR from pBL67
    pBL91pBL100 targeted to grdAThis study
    pBL93pBL68 containing prdRThis study
    pBL100pBL68 containing un-targeted groupII intron–TargeTron vectorThis study
    pCE240Derivative of pJIR750ai (Sigma-Aldrich), Tmr42
    pCR2.1Invitrogen
    pMC123Ampr Tcr44
    pRK24Tra+ Mob+ Ampr Tcr45