Table 3

Doubling times of wild-type and mutant strains in TY medium with proline and glycinea

StrainGenotypeDoubling time (min) in:
TY medium aloneTY medium + prolineTY medium + glycine
JIR8094Wild type55.41 ± 2.0041.51 ± 0.1856.51 ± 2.72
LB-CD4prdB::erm57.43 ± 2.4355.81 ± 0.6854.43 ± 2.57
LB-CD12grdA::erm57.44 ± 3.0039.11 ± 0.5758.67 ± 1.57
LB-CD8prdR::erm63.83 ± 6.9970.41 ± 4.1364.15 ± 4.63
  • a Strains were grown in TY medium alone or supplemented with 30 mM l-proline or 30 mM glycine. Doubling time was calculated during a 60-min period starting at 120 min of growth; values are means ± standard deviations.