Table 1

Bacterial strains and plasmids used in this study

Strain or plasmidCharacteristicsSource or reference
Escherichia coli strains
    W3110 (wild type)F IN(rrnD-rrnE)176
    AR3289 (FabZ*)F IN(rrnD-rrnE)1 sfhC21 zad220::Tn1077
    CF5802 (Δppk-ppx)F λ ΔilvG rfb-50 rph-1 Δppk-ppx::Kan58
    CF11608 (SpoT_E319Q)F λ ΔilvG rfb-50 rph-1 spoT(E319Q)aR. Harinarayanan and M. Cashel
    CF1652 (ΔrelA251)F λ ΔilvG rfb-50 rph-1 ΔrelA251::Kan32
    CF1693 (ΔrelAspoT)F λ ΔilvG rfb-50 rph-1 ΔrelA251::Kan ΔspoT207::Cm32
    RH99 (ΔrpoS359)F araD139 Δ(argF-lac)U169 rpsL150 ptsF25 flbB5301 rpsR deoC relA1 Φ(csi-5::lacZ) (λplac Mu55) rpoS359::Tn1078
Plasmids
    pBAD24Amprb; PBAD; araC79
    pBO110pBAD24 derivative coding for E. coli LpxC6
  • a Mutation results in loss of the (p)ppGpp synthase function of SpoT but does not affect the hydrolase function.

  • b Ampr, ampicillin resistance.