Table 2

Growth conditions, the resulting generation times, and LpxC half-lives for degradation experiments in E. coli WTa

Generation time (min)MediumTemp (°C)LpxC half-life (min)
333M9269
278M9269
263M9378
263M9269
250M9269
217M93711
208M9409
125LB304
114M93711
100LB306
100LB308
94LB2610
86M9408
81LB3717
63M9 + 10% LB3729
55LB3720
55LB4525
54LB3813
53M9 + 50% LB3910
48LB3929
47M9 + 20% LB3721
45LB4246
44M9 + 50% LB3938
41LB4531
38LB3740
37LB4539
35LB4032
33LB4560
32M9 + 50% LB39120
32LB4080
30LB40120
  • a Conditions used for the representative blots shown in Fig. 1A are highlighted in gray.