TABLE 1

Strains and primers used in this study

Strain or primerRelevant characteristic(s) or sequence (5′–3′)aSource or reference
Strains
    E. coli
        DH5αF ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK mK+) phoA supE44 λ thi-1 gyrA96 relA1Stratagene
        BL21-CodonPlus(DE3)–RILE. coli B F ompT hsdS(rB mB) dcm+ Tetr gal λ(DE3) endA Hte [argU ileY leuW; Camr]Agilent Technologies
    T. kodakarensis
        KU216ΔpyrF34
        DADΔpdaD ΔpyrF32
        DBP1ΔpdaD ΔbpsA::pdaD ΔpyrFThis study
Primers
    tk0545-FwGGAAAACCATATGATGGCTGGAAAGGTCAGThis study
    tk0545-RvGGAATTCTCAGAAGACGTTTACCTTGTCCTThis study
    tk0548-FwGGAAAACCATATGATGGCGCTGAGCGACAGThis study
    tk0548-RvGGAATTCTTAAACGAGCTTTTTCTCCTTCAThis study
    tk0967-FwAAAAAAACATATGATGAGGATCGAAAGGCTGAAThis study
    tk0967-RvAGAATTCTCAAATAAGCTCCCTCTCCGThis study
    tk1691-FwAAAAAAACATATGATGAGGGAGATAATTGAGAGThis study
    tk1691-RvAGAATTCTCAGGTAGTCGAGCTCTCCTThis study
    tk1691-up1000-FwTTCCCCTTCTCATCGACATCThis study
    tk1691-down1000-RvAATCTAGAACGTCTCCCAGATCAGCThis study
    tkpdaD-Fw1AAGGATCCCGAGAATGATGTTTTAGCThis study
    tkpdaD-Rv2GACTAGTTCAGTAGGGGAACATGACThis study
    inv-TK1691-FwGACTAGTGCCTTTCTGATTTATTTTThis study
    inv-TK1691-RvAAGGATCCATCTCACACCTCCAGAAGThis study
    tk1691_out_1GTTCTTATTTTTTTGTTTGTThis study
    tk1691_out_2AAAAAAAATTAATTAGCCACGCACCCCCTAGGGThis study
    tk1691_in_1GAGGCTCGCGAAGAAGAAGGThis study
    tk1691_in_1ACGAATATCGCGCCCTCCTCThis study
  • a Underlined sequences indicate restriction enzyme sites.