TABLE 1

Bacterial strains and plasmids used in this study

Bacterial strain or plasmidCharacteristic(s)aSource
Strains
    Enterococcus faecalis W11Wild typeIPOD
    Enterococcus faecalis Δpfl mutantΔpflB::TcrThis study
    Escherichia coli DH10BF mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara leu)7697 galU galK λ rpsL endA1 nupGLaboratory stock
Plasmids
    pHSG396Cloning vector, CmrTaKaRa
    pHY300PLKShuttle vector for E. coli and Bacillus subtilis, Apr TcrTaKaRa
    pAM401Shuttle vector for E. coli and E. faecalis, Cmr TcrATCC
    pAM-lacZControl plasmid of reporter assay for E. faecalis, CmrThis study
    pAM-PldhL1-lacZReporter assay plasmid of ldhL1 gene using β-galactosidase for E. faecalis, CmrThis study
    pAM-PpdhAB-lacZReporter assay plasmid of pdhAB gene using β-galactosidase for E. faecalis, CmrThis study
    pAM-PadhE-lacZReporter assay plasmid of adhE gene using β-galactosidase for E. faecalis, CmrThis study
    pAM-PpflB-lacZReporter assay plasmid of pflB gene using β-galactosidase for E. faecalis, CmrThis study
  • a Ap, ampicillin; Cm, chloramphenicol; Tc, tetracycline.