TABLE 4

Percent abundances of PE, PG, CL, and PA determined by LC-MS of minicell-producing mutants of wild-type (WM1032), ΔclsABC (POM10), and ΔpgsA (UEM543) E. coli strains

Genotype of strainMean % abundance (SD)
PEPGCLPA
Whole cellsPolesWhole cellsPolesWhole cellsPolesWhole cellsPoles
Wild type93.4 (±0.6)72 (±7)4.4 (±0.5)12.0 (±0.4)2.0 (±0.2)16 (±8)0.14 (±0.07)0.4 (±0.4)
ΔclsABC93.2 (±0.6)73 (±10)6.1 (±0.9)27 (±10)0.7 (±0.3)0.3 (±0.3)0.07 (±0.04)0.4 (±0.4)
ΔpgsA97.2 (±0.3)98.9 (±0.3)0.01 (±0.01)0.04 (±0.01)0.24 (±0.01)0.37 (±0.09)2.6 (±0.2)0.7 (±0.3)