TABLE 1

Strains and plasmids used in this study

Strain or plasmidRelevant genotype or other characteristicsAntibiotic resistanceaSource or reference
E. coli strains
    CV1Wild type for chemotaxis; equivalent to RP437 thr-1(Am) leuB6 his-4 metF159(Am) rpsL136 [thi-1 ara-14 lacY1 mtl-1 xyl-5 eda tonA31 tsx-78]Str16
    CV5CV1 Δtsr thr+Str50
    CV12CV1 (tar-tap5201 trg::Tn10 eda+Str, Tet50
    CV16CV1 Δtsr thr+ (tar-tap5201 trg::Tn10 eda+Str, Tet50
    BW25113 ΔtynABW25113 tynAΩKanKan17
    BW25113 ΔfeaBBW25113 feaBΩKanKan17
    BW25113 ΔqseCBW25113 qseCΩKanKan17
    SP101CV1 ΔtynAStr, KanThis study
    SP102CV1 ΔfeaBStr, KanThis study
    SP103CV1 ΔqseCStr, KanThis study
    UU2641RP437 serB::kan-ccdBStr, KanJ. S. Parkinson, personal communication
    UU2375RP437 tsr-R69E (tar-tap5201 (aer1 ygjG::Gm (trg4543StrParkinson, personal communication
    UU2376RP437 tsr-R69E (tar-tap5201 (aer1 ygjG::Gm (trg4543StrParkinson, personal communication
    TAMU100CV1 serB::kan-ccdBThis study
    TAMU101CV1 tsr-R69EThis study
    TAMU102CV1 tsr-T156KThis study
    TAMU104TAMU101/pRR53-tsrR69EThis study
    TG1supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5 (rK mK) F′ [traD36 proAB+ lacIq lacZΔM15]Stratagene
Plasmids
    pCM18GFP expression vectorErm51
    pDS-Red ExpressRFP expression vectorAmpClontech
    pRR53-tsrR69EExpresses Tsr-R69E under the control of IPTGAmp36
  • a Abbreviations: Str, streptomycin; Tet, tetracycline; Kan, kanamycin; Erm, erythromycin; Cm, chloramphenicol; Amp, ampicillin.