TABLE 1

Bacterial strains used in this study

StrainBackgroundDescriptionGenotypeReference
RN42208325-4Restriction-deficient cloning host54
RN9011RN4220RN4220 containing the pRN7023 vector encoding the SaPI integrase55
VJT 1.01NewmanWild typeWild type56
VJT 34.79VJT 1.01Newman wild type containing the ssl7 promoter driving gfp expression integrated into the chromosomeWild typeThis study
VJT 9.98VJT 1.01Transduction of rot::Tn917 from RN10623 into VJT 1.01rot::Tn91724
VJT 34.84VJT 9.98Newman rot::Tn917 containing the ssl7 promoter driving gfp expression integrated into the chromosomerot::Tn917This study