TABLE 2

Plasmids used in this study

PlasmidDescriptionSource or reference
    pMP716Kmr, E. coli-F. tularensis shuttle vector, higher copy no.35
    pMP780Kmr, sacB suicide vector derived from pMP590 with Pdnak-sacB58
    pMP789Kmr, pMP780 suicide vector bearing Δlnt alleleThis work
    pMP790Kmr, blaB integration vector based on pMP741 with PrpsL-lacZ58
    pMP812Kmr, 192 bp smaller, improved sacB suicide vector derived from pMP78058
    pMP822Hygr, E. coli-F. tularensis shuttle vector stable with PblaB-mcs (multiple-cloning site)35
    pMP823Hygr, E. coli-F. tularensis shuttle vector unstable with PblaB-mcs35
    pMP874Hygr, pMP822 stable PblaB-lnt+This work
    pMP882Kmr, pMP716 higher copy no., PblaB-lsp+This work
    pMP872Kmr, E. coli vector encoding full-length LpnA with C-terminal c-Myc tagThis work
    pMP902Kmr, pMP812 suicide vector bearing ΔlolCD alleleThis work
    pMP942Kmr, pMP812 suicide vector bearing ΔbamD alleleThis work
    pMP985Hygr, pMP823 E. coli-F. tularensis shuttle vector bearing PrpsL-lacZ from pMP790This work
    pMP992Hygr, pMP985 unstable PblaB-lolCD+This work
    pMP1003Hygr, pMP985 unstable PblaB-bamD+This work
    pMP1181Kmr, E. coli-F. tularensis shuttle vector, higher copy no., PrpsLlpnA102 (“short” c-Myc-tagged reporter)