TABLE 1

Strains and plasmids used in this study

Strain or plasmidDescriptionReference
S. meliloti strains
    A2102Rm1021 nodD1::Tn5 nodD2::Tmr nodD3::Tn5-233; Spr Tmr Nmr Gmr Smr16
    A2105Rm1021 nodD1::Tn5 nodD2::Tmr nodD3::Tn5-233 nodC::lacZ; Spr Tmr Nmr Gmr Smr16
    CL150Rm1021 pstC+ ecfR1+86
    DW386Rm1021 nodF::uidA; Nmr Smr129
    EC69Rm1021 chvI K214T hisB; Hyr Smr43
    EC176Rm1021 hisB; Hyr Smr43
    JM57Rm1021 nodC::lacZ; Spr Smr18
    JM61Rm1021 nodD1::lacZ; Spr Smr18
    MB669Rm1021 flaC::uidA; Spr Smr80
    MB670Rm1021 mcpX::uidA; Spr Smr80
    MB671Rm1021 mcpU::uidA; Spr Smr80
    MB672Rm1021 SMc00888::uidA; Spr Smr80
    MB673Rm1021 SMc00887::uidA; Spr Smr80
    MB705Rm1021 ΔsyrA; BglII-ClaI replaced with Nmr cassette; Nmr Smr94
    MB709Rm1021 ΔsyrA exoR95::Tn5-233 pho-1; Nmr Spr Gmr SmrThis study
    MB710Rm1021 ΔsyrA exoS96::Tn5-233 pho-1; Nmr Spr Gmr SmrThis study
    MB735Rm1021 ΔnodD1; Nmr SmrThis study
    MB736Rm1021 ΔnodD2; Spr SmrThis study
    MB771Rm1021 ΔnodD3; Hyr SmrThis study
    MB781Rm1021 ΔnodD1D2D3 nodC::lacZ; Hyr Nmr Spr SmrThis study
    MB1002CL150 ΔnodD1D2D3; Hyr Nmr Spr SmrThis study
    MB1003CL150 ΔnodD1D2D3 nodC::lacZ; Hyr Nmr Spr SmrThis study
    MB1031CL150 ΔnodD3 nodD1::lacZ nodF::uidA; Hyr Nmr Spr SmrThis study
    MB1035CL150 ΔsyrA; Nmr SmrThis study
    MB1058Rm1021 with single crossover of pMB814; Gmr Nmr SmrThis study
    MB1061/pDW199ΔchvI strain carrying pDW199; Tcr Nmr SmrThis study
    Rm1021Wild-type SU47; Smr130
    Rm7210Rm1021 exoY210::Tn5; Nmr Smr33
    Rm7225Rm1021 exoH225::Tn5; Nmr Smr131
    Rm8264exoA264::TnphoA; Nmr Smr89
    Rm8270exoP270::TnphoA; Nmr Smr89
    Rm8274exoT274::TnphoA; Nmr Smr89
    Rm8286exoV2::Tn5; Nmr Smr87
    Rm8295Rm1021 exoR95::Tn5-233 pho-1; Spr Gmr Smr132
    Rm8296Rm1021 exoS96::Tn5-233 pho-1; Spr Gmr Smr132
    Rm8332exoQ332::Tn5; Nmr Smr131
    Rm8337exoU337::Tn5; Nmr Smr88
    Rm8341exoZ341::Tn5; Nmr Smr133
    Rm8369exoF369::TnphoA; Nmr Smr89
    Rm8416exoN416::Tn5; Nmr Smr131
    Rm8457exoM457::Tn5; Nmr Smr131
    Rm8468exoP468::Tn5; Nmr Smr131
    Rm8476exoK476::Tn5; Nmr Smr89
E. coli DH5αendA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 deoR128
Plasmids
    pBluescript SK(+/−)ColE1; cloning vector; Apr134
    pDW83pMS03 exoR; Spr43
    pDW199PchvI-chvI in IncP broad-host-range vector; Tcr43
    pE43pTE3 nodD1; Tcr13
    pE65pTE3 nodD3; Tcr13
    pJQ200SKsacB vector; P15a ori, does not replicate in S. meliloti; Gmr66
    pMB89pTE3 syrA; Tcr26
    pMB90pTE3 derivative; same as pMB89, except with insert in opposite orientation; Tcr26
    pMB387Nmr and Kmr in pBluescript SK(−); cassette excised as BamHI-HindIIIThis study
    pMB814pJQ200SK ΔchvI; Nmr KmrThis study
    pMB763pJQ200SK with ΔnodD1 cloned in SpeI/XhoI sites; Nmr KmrThis study
    pMB764pJQ200SK with ΔnodD2 cloned in XbaI/SpeI sites; SprThis study
    pMB769pJQ200SK with ΔnodD3 cloned in ApaI/XbaI sites; HyrThis study
    pMS03pBBR broad-host-range vector; trp promoter; Spr52
    pRK290IncP broad-host-range vector; Tcr135
    pRK600ColE1; provides RK2 transfer functions; Cmr63
    pS73pTE3 syrM; Tcr23
    pTE3pRK290 with trp promoter; confers strong constitutive expression in S. meliloti; Tcr136
    pVO122Spr in pBluescript SK(+); cassette excised as BamHI-XhoI137
    pVO205Hyr cassette in in pBluescript SK(+); cassette excised as BamHI137