TABLE 2

Strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)aSource or reference
E. coli TOP10Cloning strainInvitrogen
H. pylori
    43504Parental strainATCCb
    ΔureB mutantΔureB::KSF Kanr SucsThis study
    ΔureAB mutantΔureAB::cat Cmr15
    ureBH136A strainCodon for His136 replaced by AlaThis study
    ureBH138A strainCodon for His138 replaced by AlaThis study
    ureBK219A strainCodon for Lys219 replaced by AlaThis study
Plasmids
    pBluescript KS(+)Cloning vector; AprStratagene
    pKS-ureBureB cloned into the KpnI-SacI site of pBluescript KS(+)This study
    pKS-ureB::KSFKSF cassette cloned into the BamHI site of pKS-ureBThis study
    pKS-ureBH136ApKS-ureB with His136 replaced by AlaThis study
    pKS-ureBH138ApKS-ureB with His138 replaced by AlaThis study
    pKS-ureBK219ApKS-ureB with Lys219 replaced by AlaThis study
  • a Cm, chloramphenicol; Ap, ampicillin; Kan, kanamycin; Suc, sucrose; KSF, Kanr-sacB-PflaA (62).

  • b American Type Culture Collection, Manassas, VA.