Table 2.

Plasmids used in this study

PlasmidParentRelevant genotype (cpe promoter region present)Source or reference
 pCRIIInvitrogen
 pBS−Stratagene
 pBluescript SK−Stratagene
 pSM100pBluescript SK−NCTC 10240 cpe promoter22
 pSM101pBluescript SK−NCTC 8798 cpepromoter22
 pJIR750J. Rood (2)
 pSM104pJIR750φ(cpeNCTC 10240-gusA) (−481 to +41)22
 pSM123pSM104φ(cpeNCTC 10240-gusA) (−296 to +41)This study
 pSM127pSM104φ(cpeNCTC 10240-gusA) (−121 to +41)This study
 pSM129pSM104φ(cpeNCTC 10240-gusA) (−56 to +41)This study
 pSM145pSM104φ(cpeNCTC 10240-gusA) (−183 to +41)This study
 pSM151pBS−cpe terminator regionThis study
 pSM154pBS−NCTC 8798 cpe promoter; in vitro transcription vectorThis study
 pSM155pBS−NCTC 10240cpe promoter; in vitro transcription vectorThis study
 pSM164pSM104φ(cpeNCTC 10240-gusA)This study
 pSM165pSM104φ(cpeNCTC 10240-gusA)This study
 pSM170pSM104φ(cpeNCTC 10240-gusA)This study
 pSM183pSM104φ(cpeNCTC 10240-gusA)This study