Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)aSource
Escherichia coliDH5αFendA1 hrdR17(Rk mK+)recA114
Ralstonia solanaceaum
 K60Wild type26
 S49EPS;epsI::lacZ fusion; Kanr29
 S70EPS;epsI::lacZ fusion; Kanr29
 S90EPS;rgnII::lacZ fusion; Kanr29
 S112EPS;vsrB::lacZ orvsrC::lacZ fusion; Kanr29
 S50EPS;ops::lacZ fusion; Kanr29
 K60::epsRΩEPS+; EpsRThis work
 S49::epsRΩEPS;epsI::lacZ fusion; epsRKanr SmrThis work
 S70::epsRΩEPS;epsI::lacZ fusion; epsRKanr SmrThis work
 S90::epsRΩEPS;rgnII::lacZ fusion; epsRKanr SmrThis work
 S112::epsRΩEPS;vsrB::lacZ orvsrC::lacZ fusion epsRKanr SmrThis work
 S50::epsRΩEPS;opsG::lacZ fusion; epsRKanr SmrThis work
 S49::epsRD-A 1EPS;epsI::lacZ fusion; epsREpsRD-A+ Kanr SmrThis work
 S49::epsRD-A 10EPS;epsI::lacZ fusion; epsREpsRD-A+ Kanr SmrThis work
Plasmids
 pLAFR3Tcr25
 pGL10KanrY. Brun
 pGL10lacZPromoterless lacZ gene from mini-Tn5::lacZ cloned into pGL10; KanrThis work
 pGL10ΩΩ fragment from pHP45Ω cloned into the BglII site of pGL10; Kanr SmrThis work
 pGepsI::lacZNucleotides 1 to 260 of theepsI promoter cloned into the EcoRI andBamHI sites of pGL10lacZ; KanrSmrThis work
 pGepsR::lacZNucleotides 25 to 365 ofepsR cloned into the EcoRI and KpnI sites of pGL10lacZ; Kanr SmrThis work
 pGopsG::lacZ360-bp fragment containing theopsG promoter cloned into the EcoRI site of pGL10lacZ; Kanr SmrThis work
 Mini-Tn5lacZ2AmprKanr28
 pHP45ΩSmr AmprY. Brun;34
 pepsRΩΩ cassette cloned into theSmaI site of epsR contained on pET11epsRThis work
 pTAepsINucleotides 1 to 260 of epsI cloned into PCRII as a PCR product; Kanr SmrThis work
 pKLS44Ampr21
 pET11epsRAmpr21
 pGepsRNucleotides 25 to 1290 of epsR cloned into pGL10This work
 pepsRNucleotides 25 to 1142 ofepsR cloned into pLAFR3; TetrThis work
 pepsRD-ANucleotides 25 to 1142 of epsRD-A cloned into EcoRI and HindIII sites of pLAFR3; TetrThis work
 pBepsRD-ANucleotides 25 to 1142 of epsRD-A cloned in pBSKSThis work
 pepsRD-AΩNucleotides 286 to 1142 of epsRD-Acloned in pBSKSThis work
 pKL4Tcr16
 pL112Tcr29
 pL90Tcr29
  • a Ampr, ampicillin resistance; Smr, streptomycin resistance; Tcr, tetracycline resistance; Kanr, kanamycin resistance.