Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristicsaReference or source
E. coli strain
 INVαF′F′ endA1 recA1 hsdR17(rKmK+) supE44 thi-1 gyrA96 relA1φ80lacZΔM15 Δ(lacZYA-argF)U169 λInvitrogen, San Diego, Calif.
S. mutans strains
 UA55Tcr, Tn916 inserted in DGK, Mut2a
 T8Wild type, Mut+23
 T8-1Tcr, Tn916 inserted in DGK, MutThis study
 T8-1TTcrEmr, T8-1 transformed with pCBD5, Mut+This study
S. sobrinus strain
 OMZ176Mutacin-sensitive indicator5
Plasmids
 pUC19AprLacZa36
 pCRIIAprKmr, cloning vectorInvitrogen
 pNoTA/T7Apr, cloning vector5 Prime → 3 Prime, Inc., Boulder, Colo.
 pVA891EmrCmr, shuttle plasmid18
 pCBD1Apr Kmr, pCRII-containing 2.5-kb TnLO-2 SSP-PCR fragment containing sgpThis study
 pCBD2Apr Kmr, pCRII with 0.8-kb Tn-R-O SSP-PCR fragment containing truncated dgk and partorf3This study
 pCBD3AprKmr, pCRII with 0.9-kb SSP-PCR fragment containing the promoter region and 5′ end of orf3This study
 pCBD4Apr, pNoTA/T7-containing 0.6-kb PCR fragment containing complete dgk coding regionThis study
 pCBD5Emr Cmr, pVA891-containing 0.6-kb PCR fragment containing complete dgk coding regionThis study
 pCBMAApr, pNoTA/T7-containing 2.2-kb PCR fragment containing completemutA coding region5a
  • a Abbreviations: Ap, ampicillin; Em, erythromycin; Km, kanamycin; Tc, tetracycline; Mut, mutacin negative; Mut+, mutacin positive.