Table 1.

Bacterial strains and plasmids used

Strain or plasmidRelevant propertiesaSource or reference
E. coli strains
 BL21(DE3)F ompT hsdS B(rB mB ) gal dcm (DE3)Novagen
 SY327(λpir)Δ(lac-pro)argE(Am) rif nalA recA56 λpir 34
 DH5α end-1 hsdR17(rK mK +)supE44 thi-1 recA1 gyrA (Nalr) relA1Δ(lacIZYA-argF)U169 deoR [φ80dlacΔ(lacZ)M15] 20
 XL1-Blue end-1 hsdR17(rK mK +) supE44 thi-1 λ recA1 gyrA96 (Nalr) relA1lac) [F+ proAB+lacI q ZΔM15::Tn10(Tcr)]Stratagene
Y. pestisstrainsb
 KIM5-3001Smr pCD1 (Lcr+), pPCP1, pMT1 32
 KIM5-3001.2Smr pCD1 lcrD(Δ192–343), pPCP1, pMT1 44
 KIM5-3001.2.1Smr pCD1lcrD(Δ192–343) yopD(Δ1–305), pPCP1, pMT1This study
 KIM5-3001.16Smr pCD1yopD(Δ1–305), pPCP1, pMT1This study
 KIM8-3002Smr pCD1 (Lcr+), Pla, pMT1 38
 KIM8-3002.2Smr pCD1 yopD(Δ1–305), Pla, pMT1This study
Plasmids
 pUK4134Suicide vector, Apr 54
 pAW1021.64-kb fragment of pCD1 containing ΔyopD (Δ1–305) cloned into pUK4134This study
 pTrcH5 lcrH cloned into pTrc99A (Pharmacia Biotech) 12
 pET-24bHis tag fusion expression vector, Kmr Novagen
 pAW111 yopD cloned into pET-24bThis study
 pBAD33Expression vector (arabinose inducible), Cmr 16
 pAW161 yopD cloned into pBAD33This study
 pAW162 lcrQ cloned into pBAD33This study
 pProEX-1His tag fusion expression vector, Apr GibcoBRL
 pAW171 yopD cloned into pProEX-1This study
 pGEX-3XGST fusion expression vector, Apr Pharmacia Biotech
 pWGQ lcrQcloned into pGEX-3XThis study
  • a Numbers in the parentheses in the gene designations represent the amino acids deleted from the encoded protein.

  • b All Y. pestis strains are Pgm (60). Native plasmids of Y. pestis include the LCR plasmid pCD1 (9, 15), the Pla-encoding pPCP1 (57), and pMT1, which encodes the capsular protein (49).