Table 1.

Summary of the 16S rDNA sequences identified in OP sediment from three clone libraries, OPB, OPS, and OPT

Type sequenceaOther sequenced representative(s)bNo. of clonescBacterial divisionDatabase matches (≥98% identity)Inferred metabolismInferred thermophilyd
OPT51α-Proteobacteria
OPB30S63, S83, S130, S14014β-ProteobacteriaHot spring clone OS type G
OPS1222β-Proteobacteria
OPB37S92Be2β-Proteobacteria
OPB33B8, B78e, S27, S105, T7729δ-ProteobacteriaSulfate reduction
OPB55S22, S86Ae6δ-ProteobacteriaSulfate reduction?
OPT23T472δ-ProteobacteriaSulfate reduction?
OPB161δ-ProteobacteriaSulfate reduction?
OPS961δ-ProteobacteriaSulfate reduction
OPT561δ-ProteobacteriaSulfate reduction?
OPB67A1Nitrospira group
OPT35T372NitrospiragroupThermodesulfovibrio yellowstoniiSulfate reduction
OPB32Acidobacteriumgroup+
OPB351Verrucomicrobium group
OPB88S12BCFf group+
OPB731BCF group
OPB56S133Be3BCF–green-sulfur bacteria
OPS77T9e2Green-sulfur bacteria
OPS1851Green-sulfur bacteria
OPB41S17, S102, T96Ae5High-G+C gram positives+
OPT291High-G+C gram positives+
OPB19B31, B32, S8Ae, S15, S25, T6027Thermus-Deinococcus groupThermussp. strain YSPIDOrganotrophy+
OPS1281OP11
OPS136S67, S685OP11
OPB922OP11
OPB12B11, S8Be, S69Ae, S12417Green nonsulfur bacteria+
OPB65S54Be, S57, S121, T868Green nonsulfur bacteria
OPT90eS143Be2Green nonsulfur bacteria
OPB9B342Green nonsulfur bacteria
OPB14S143Ae2OP1+
OPB40S32OP4
OPS107B60e, S51, S54Ae, S148, S163, T69e, T76e16OP5
OPB17B6, T495Planctomycetales
OPB95S35Be, S88, S92Ae, S150, S12, T312OP8
OPB5B23, S19Ae, S377OP8
OPB7B85e, S49Ae, S6611Thermotogales+
OPS196S69Be2Thermotogales
OPS61e1Dictyoglomus group
OPS1451DictyoglomusgroupDictyoglomus thermophilumOrganotrophy
OPS152e1OP6
OPS133Ae1OP7
OPB46B47, S44, S118, S139Ae9OP9+
OPB72e1OP9
OPB541OP12+
OPB21OP3
OPB80B90, S1425OP10+
OPB501OP10+
OPT4T532ThermodesulfobacteriumgroupSulfate reduction
OPS71ThermodesulfobacteriumgroupSulfate reduction
OPB451ThermodesulfobacteriumgroupSulfate reduction+
OPB251OP2
OPB13S2, S35Ae, S55, T11e, T20e80AquificalesHot spring clone pBLACKHydrogen oxidation
OPS1322AquificalesCalderobacterium hydrogenophilumHydrogen oxidation+
OPS165T142AquificalesHot spring clone EM17Hydrogen oxidation+
 Total129312
  • a Both strands were sequenced and used as representatives in phylogenetic analyses.

  • b Shorthand clone name which omits OP used.

  • c With ≥98% identity to the type sequence, based on direct sequence comparison or inferred from RFLP patterns.

  • d Positives are rDNAs with G+C content of >60%.

  • e Short sequences ranging from 300 to 1,350 nt, due to mispriming events during PCR. 5′ and 3′ parent fragments of chimeric sequences are designated A and B, respectively.

  • f BCF,Bacteroides-Cytophaga-Flexibacter.