Table 1.

Summary of the 16S rDNA sequences identified in OP sediment from three clone libraries, OPB, OPS, and OPT

Type sequenceaOther sequenced representative(s)bNo. of clonescBacterial divisionDatabase matches (≥98% identity)Inferred metabolismInferred thermophilyd
OPB30S63, S83, S130, S14014β-Proteobacteria Hot spring clone OS type G
OPB37S92Be 2β-Proteobacteria
OPB33B8, B78e, S27, S105, T7729δ-Proteobacteria Sulfate reduction
OPB55S22, S86Ae 6δ-Proteobacteria Sulfate reduction?
OPT23T472δ-Proteobacteria Sulfate reduction?
OPB161δ-Proteobacteria Sulfate reduction?
OPS961δ-Proteobacteria Sulfate reduction
OPT561δ-Proteobacteria Sulfate reduction?
OPB67A1 Nitrospira group
OPT35T372 Nitrospiragroup Thermodesulfovibrio yellowstonii Sulfate reduction
OPB32 Acidobacteriumgroup+
OPB351 Verrucomicrobium group
OPB88S12 BCFf group +
OPB731 BCF group
OPB56S133Be 3 BCF–green-sulfur bacteria
OPS77T9e 2Green-sulfur bacteria
OPS1851Green-sulfur bacteria
OPB41S17, S102, T96Ae 5High-G+C gram positives+
OPT291High-G+C gram positives+
OPB19B31, B32, S8Ae, S15, S25, T6027 Thermus-Deinococcus group Thermussp. strain YSPIDOrganotrophy+
OPS136S67, S685OP11
OPB12B11, S8Be, S69Ae, S12417Green nonsulfur bacteria+
OPB65S54Be, S57, S121, T868Green nonsulfur bacteria
OPT90e S143Be 2Green nonsulfur bacteria
OPB9B342Green nonsulfur bacteria
OPB14S143Ae 2OP1+
OPS107B60e, S51, S54Ae, S148, S163, T69e, T76e 16OP5
OPB17B6, T495 Planctomycetales
OPB95S35Be, S88, S92Ae, S150, S12, T312OP8
OPB5B23, S19Ae, S377OP8
OPB7B85e, S49Ae, S6611 Thermotogales +
OPS196S69Be 2 Thermotogales
OPS61e 1 Dictyoglomus group
OPS1451 Dictyoglomusgroup Dictyoglomus thermophilum Organotrophy
OPS152e 1OP6
OPS133Ae 1OP7
OPB46B47, S44, S118, S139Ae 9OP9+
OPB72e 1OP9
OPB80B90, S1425OP10+
OPT4T532 ThermodesulfobacteriumgroupSulfate reduction
OPS71 ThermodesulfobacteriumgroupSulfate reduction
OPB451 ThermodesulfobacteriumgroupSulfate reduction+
OPB13S2, S35Ae, S55, T11e, T20e 80 Aquificales Hot spring clone pBLACKHydrogen oxidation
OPS1322 Aquificales Calderobacterium hydrogenophilum Hydrogen oxidation+
OPS165T142 Aquificales Hot spring clone EM17Hydrogen oxidation+
  • a Both strands were sequenced and used as representatives in phylogenetic analyses.

  • b Shorthand clone name which omits OP used.

  • c With ≥98% identity to the type sequence, based on direct sequence comparison or inferred from RFLP patterns.

  • d Positives are rDNAs with G+C content of >60%.

  • e Short sequences ranging from 300 to 1,350 nt, due to mispriming events during PCR. 5′ and 3′ parent fragments of chimeric sequences are designated A and B, respectively.

  • f BCF,Bacteroides-Cytophaga-Flexibacter.