Table 1.

Function of virB2 to virB11 mutants in RSF1010 mobilization

StrainaRelevant characteristicsMobilization of pML122ΔKmb
A348Wild type1.3 × 10−3
A348ΔB2ΔvirB2 <6.3 × 10−7
A348ΔB3ΔvirB3 <7.6 × 10−7
At12031 virB3::Tn5virB(2108)<4.2 × 10−7
At12044 virB4::Tn5virB(3549)<6.3 × 10−7
A348ΔB5ΔvirB5 <8.8 × 10−7
A348ΔB6ΔvirB6 <5.3 × 10−7
At12063 virB6::Tn5virB(6125)<3.3 × 10−7
A348ΔB7ΔvirB7 <7.1 × 10−7
A348ΔB8ΔvirB8 <7.0 × 10−7
At12081 virB8::Tn5virB(6779)<5.8 × 10−7
Ax42 virB9::Tn5virB(7878)<1.0 × 10−6
A348ΔB10ΔvirB10 <9.6 × 10−7
At12101 virB10::Tn5virB(8346)<4.5 × 10−7
At12102 virB10::Tn5virB(8416)<5.1 × 10−7
At10011ΔvirB11 <1.1 × 10−6
  • a Construction of Tn5virBstrains is described in the text, except for that of Ax42 (11), which was obtained from A. Binns. Numerical designation following Tn5virB refers to site of insertion according to the DNA sequence published by Ward et al. (26). All A348ΔB strains (4) were obtained from P. Christie. At10011 was from K. Stephens (24).

  • b Conjugation assays were done as previously described (14). Frequencies of transfer of pML122ΔKm to recipient UIA143 are expressed as transconjugants per input donor. Data are the means of triplicates from a single assay. Three independent experiments were done, with similar results.