Table 2.

Purification of enoyl-CoA hydratase from E. coli BL21(DE3)/pETNB3

Purification stepVol (ml)ProteinTotal activitya (U)Sp acta (U/mg)Yield (%)Purification (fold)
mgmg/ml
None (crude enzyme)10101  10.11.7 × 1051.7 × 103
Q-Sepharoseb521.64.31.1 × 1055.1 × 103653
  • a Enoyl-CoA hydratase activity toward crotonyl-CoA (0.25 mM).

  • b Enzyme was concentrated and desalted after elution from a Q-Sepharose column.