Table 1.

Bacterial strains and plasmids used

Strain or plasmidGenotype and/or phenotypeReference(s) or source
Strains
E. coli
 DH5α endA1 hsdR17(rK mK +) supE44 thi-1 recA1 gyrArelA1 Δ(lacZYA-argF)U169(φ80lacZΔM15) 30
 PhiPsts 19
 S17-1 λ pir+ tra+ 54,39
Y. enterocolitica
  WA-CPlasmidless derivative of strain WA-134, serotype O8. Spontaneous Nalr mutant 31
  WA-C irp2 WA-C with pGPIRP2 inserted intoirp2 geneThis study
  WA-CSSpontaneous Smr mutant derived from WA-CThis study
  WAfyuA fyuA mutant of WA-C, Pstr 44
  WA-CSirp1::Kanr Derivative of WA-CS, Nalr Smr Kmr This study
  8081Serotype O8 42
  H1852fyuA Derivative of WA-C, fur fyuA mutant of WA-CThis study
  Y5.27Clinical isolate, serotype O5.27 31
  Y-96-CClinical isolate, serotype O9 31
  Y-108-CClinical isolate, serotype O3 31
Y. pseudotuberculosis
  346 irp2+ serotype O3S. Aleksic
  201 irp2 serotype O3S. Aleksic
  PB1Serotype O1R. R. Brubaker
Y. pestis
  KIMLcrPgm+ R. R. Brubaker
  KIM Δpgm Lcr ΔPgmThis study
  KUMALcr Pgm+ R. R. Brubaker
  EV76Pesticin-producing strainR. R. Brubaker
Plasmids
 pLAFR2TcrMob+ 25
 17A11pLAFR2 carrying a 23-kb insert of WA-C chromosomal DNA, Tcr 44
 pBluescript KS IICloning vector, Apr Stratagene
 pKAS 32Cloning vector with rpsL gene 55
 pKAS-1SKanpKAS 32 containing irp1 EcoRI-2 fragment with a kanamycin cassette introduced into SalI siteThis study
 pKAS-E1KanpKAS 32 containingEcoRI/SalI fragment of irp1 with a kanamycin cassette (without transcriptional terminator) inserted into the EcoRV siteThis study
 pGP704Cloning vector, Apr 39
 pGP-CATChloramphenicol cassette inserted into PstI site of pGP704, Cmr 46
 pGPIRP2600-bp PCR fragment of irp2 inserted into pGP-CAT vectorThis study
 pUC-4KpUC vector containing kanamycin cassette from Tn903, AprKanr 60
 pSB 315Containing kanamycin cassette without transcriptional terminator, AprKanr 27
 pACYC 184Cloning vector, Cmr 11
 pCJG3.3NpACYC 184 with fyuA-gfp mut3This study
 pGFP mut3pKS with cDNA of the mutated GFP under thelac promoter 12
 SuperCos1Cloning vector lacking SalI restriction site, Apr Neor Stratagene