Table 2.

Purification steps of citrate lyase from L. mesenteroides

StepProtein (mg)Total activity (U)Sp act (U/mg)Yield (%)Purifi- cation (fold)
Crude extract3771810.481001
Protamine sulfate1821650.9191.31.8
Ammonium sulfate621302.10724.3
Hydroxylapatite4.3255.7013.511.8
Ion exchange1.31612.108.825.3