Table 1.

Total and Se-dependent glutathione peroxidase activity increased while total glutathione decreased following treatment with LoaOOHa

LoaOOH concn (mM)GPX activity (nmol min−1mg−1)bTotal glutathionecGSSG (nmol/107cells)GSH/GSSG ratio
TotalSe dependent
0123 ± 4.296 ± 104.30.566:1
0.05149139 ± 7.12.80.327:1
0.08611 ± 22419 ± 241.450.193:1
0.12593 ± 18562 ± 200.620.0511:1
0.2NDND0.430.0155:1
  • a Abbreviations: GPX, glutathione peroxidase; GSSG, oxidized glutathione; GSH, reduced glutathione; ND, not determined.

  • b Activity is expressed as nanomoles of NADPH oxidized per minute per milligram of protein. Data are means of duplicates from a representative experiment.

  • c Expressed as nanomoles of GSH plus 2× GSSG per 107 viable cells for the wild-type strain.